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anti human mouse rat prkca  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti human mouse rat prkca
    a GST-pull down assay of PSC’s whole cell lysate (WCL). The red dotted box indicated the regions for M/S analysis. b GST-Zfp260 specially enriched kinases with high HT sequest scores. c Co-IP of 293 T cell line. d Co-IP of immortalized PSCs. e Co-staining image for Zfp260 and <t>Prkca</t> in PSCs. The white dotted circle indicated the nucleus (Left). The white dotted line indicated the route for the fluorescence intensity measurements (right). f Representative images of immunofluorescence of Zfp260 with osteogenic induction, with the MFI/cytosolic MFI calculated (right). g Separation of nuclear (NE) and cytosolic extracts (CE) followed by Western blot. h In vitro Phos-Assay was performed by Phospho-PAGE. i Co-IP, Phos-PAGE, and SDS-PAGE were jointly performed in immortalized PSCs. The red dotted rectangle indicating the Y173, S182, and S197 residues. j Suggested binding mode of Zfp260 and Prkca by AlphaFold2. k The shortest distance between Zfp260-aa173 and the catalytic domain (CD) of Prkca (Z173-CD), aa182 and CD (Z182-CD), and aa197 and CD (Z197-CD) were calculated. l Co-IP was performed in immortalized PSCs, with statistical analysis (right). m Representative images of immunofluorescence of Zfp260-V5 before and after osteogenic induction (left), with the nuclear MFI/cytosolic MFI evaluated (right). n Separation of NE and CE, followed by Western blot. o Representative ARS staining images of PSCs. Scalebar: 2 mm. p–r ChIP-qPCR assays for Zfp260-V5, Brd4, and H3K27ac binding. n = 6 from 2 biological replicates. For ( a , d , e , h , i ), experiments were conducted independently 3 times, consistently producing similar results. For ( c , g , h , i , l , n , o ), n = 3 from 3 biological replicates. For ( f , m ), n = 30 from 3 biological replicates, with 10 randomly selected cells calculated per replicate. For ( e , f , m ), scale bars: 5 μm. Two-way ANOVA. Box plots display the minimum and maximum values, with the center line representing the median, and the bounds of the box representing the 25th to 75th percentiles. Other data in this figure are represented as mean ± SD. Source data and exact p values are provided in the Source Data file.
    Anti Human Mouse Rat Prkca, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+mouse+rat+prkca/PKC+alpha+Antibody+(MC5)/pmc11586402-322-75-78
    Average 92 stars, based on 21 article reviews
    anti human mouse rat prkca - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells"

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells

    Journal: Nature Communications

    doi: 10.1038/s41467-024-54640-0

    a GST-pull down assay of PSC’s whole cell lysate (WCL). The red dotted box indicated the regions for M/S analysis. b GST-Zfp260 specially enriched kinases with high HT sequest scores. c Co-IP of 293 T cell line. d Co-IP of immortalized PSCs. e Co-staining image for Zfp260 and Prkca in PSCs. The white dotted circle indicated the nucleus (Left). The white dotted line indicated the route for the fluorescence intensity measurements (right). f Representative images of immunofluorescence of Zfp260 with osteogenic induction, with the MFI/cytosolic MFI calculated (right). g Separation of nuclear (NE) and cytosolic extracts (CE) followed by Western blot. h In vitro Phos-Assay was performed by Phospho-PAGE. i Co-IP, Phos-PAGE, and SDS-PAGE were jointly performed in immortalized PSCs. The red dotted rectangle indicating the Y173, S182, and S197 residues. j Suggested binding mode of Zfp260 and Prkca by AlphaFold2. k The shortest distance between Zfp260-aa173 and the catalytic domain (CD) of Prkca (Z173-CD), aa182 and CD (Z182-CD), and aa197 and CD (Z197-CD) were calculated. l Co-IP was performed in immortalized PSCs, with statistical analysis (right). m Representative images of immunofluorescence of Zfp260-V5 before and after osteogenic induction (left), with the nuclear MFI/cytosolic MFI evaluated (right). n Separation of NE and CE, followed by Western blot. o Representative ARS staining images of PSCs. Scalebar: 2 mm. p–r ChIP-qPCR assays for Zfp260-V5, Brd4, and H3K27ac binding. n = 6 from 2 biological replicates. For ( a , d , e , h , i ), experiments were conducted independently 3 times, consistently producing similar results. For ( c , g , h , i , l , n , o ), n = 3 from 3 biological replicates. For ( f , m ), n = 30 from 3 biological replicates, with 10 randomly selected cells calculated per replicate. For ( e , f , m ), scale bars: 5 μm. Two-way ANOVA. Box plots display the minimum and maximum values, with the center line representing the median, and the bounds of the box representing the 25th to 75th percentiles. Other data in this figure are represented as mean ± SD. Source data and exact p values are provided in the Source Data file.
    Figure Legend Snippet: a GST-pull down assay of PSC’s whole cell lysate (WCL). The red dotted box indicated the regions for M/S analysis. b GST-Zfp260 specially enriched kinases with high HT sequest scores. c Co-IP of 293 T cell line. d Co-IP of immortalized PSCs. e Co-staining image for Zfp260 and Prkca in PSCs. The white dotted circle indicated the nucleus (Left). The white dotted line indicated the route for the fluorescence intensity measurements (right). f Representative images of immunofluorescence of Zfp260 with osteogenic induction, with the MFI/cytosolic MFI calculated (right). g Separation of nuclear (NE) and cytosolic extracts (CE) followed by Western blot. h In vitro Phos-Assay was performed by Phospho-PAGE. i Co-IP, Phos-PAGE, and SDS-PAGE were jointly performed in immortalized PSCs. The red dotted rectangle indicating the Y173, S182, and S197 residues. j Suggested binding mode of Zfp260 and Prkca by AlphaFold2. k The shortest distance between Zfp260-aa173 and the catalytic domain (CD) of Prkca (Z173-CD), aa182 and CD (Z182-CD), and aa197 and CD (Z197-CD) were calculated. l Co-IP was performed in immortalized PSCs, with statistical analysis (right). m Representative images of immunofluorescence of Zfp260-V5 before and after osteogenic induction (left), with the nuclear MFI/cytosolic MFI evaluated (right). n Separation of NE and CE, followed by Western blot. o Representative ARS staining images of PSCs. Scalebar: 2 mm. p–r ChIP-qPCR assays for Zfp260-V5, Brd4, and H3K27ac binding. n = 6 from 2 biological replicates. For ( a , d , e , h , i ), experiments were conducted independently 3 times, consistently producing similar results. For ( c , g , h , i , l , n , o ), n = 3 from 3 biological replicates. For ( f , m ), n = 30 from 3 biological replicates, with 10 randomly selected cells calculated per replicate. For ( e , f , m ), scale bars: 5 μm. Two-way ANOVA. Box plots display the minimum and maximum values, with the center line representing the median, and the bounds of the box representing the 25th to 75th percentiles. Other data in this figure are represented as mean ± SD. Source data and exact p values are provided in the Source Data file.

    Techniques Used: Pull Down Assay, Co-Immunoprecipitation Assay, Staining, Fluorescence, Immunofluorescence, Western Blot, In Vitro, SDS Page, Binding Assay, ChIP-qPCR

    Related Articles

    Pull Down Assay:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    Co-Immunoprecipitation Assay:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    Staining:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    Fluorescence:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    Immunofluorescence:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    Western Blot:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    In Vitro:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    SDS Page:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    Binding Assay:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).

    ChIP-qPCR:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: The tyramide reagents used in mIHC included AF350 (B40952, Invitrogen), AF488 (B40953, Invitrogen), AF546 (B40954, Invitrogen), AF594 (B40957, Invitrogen) and AF647 (B40958, Invitrogen).



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    anti human mouse rat prkca  (Novus Biologicals)
    92
    Novus Biologicals anti human mouse rat prkca
    a GST-pull down assay of PSC’s whole cell lysate (WCL). The red dotted box indicated the regions for M/S analysis. b GST-Zfp260 specially enriched kinases with high HT sequest scores. c Co-IP of 293 T cell line. d Co-IP of immortalized PSCs. e Co-staining image for Zfp260 and <t>Prkca</t> in PSCs. The white dotted circle indicated the nucleus (Left). The white dotted line indicated the route for the fluorescence intensity measurements (right). f Representative images of immunofluorescence of Zfp260 with osteogenic induction, with the MFI/cytosolic MFI calculated (right). g Separation of nuclear (NE) and cytosolic extracts (CE) followed by Western blot. h In vitro Phos-Assay was performed by Phospho-PAGE. i Co-IP, Phos-PAGE, and SDS-PAGE were jointly performed in immortalized PSCs. The red dotted rectangle indicating the Y173, S182, and S197 residues. j Suggested binding mode of Zfp260 and Prkca by AlphaFold2. k The shortest distance between Zfp260-aa173 and the catalytic domain (CD) of Prkca (Z173-CD), aa182 and CD (Z182-CD), and aa197 and CD (Z197-CD) were calculated. l Co-IP was performed in immortalized PSCs, with statistical analysis (right). m Representative images of immunofluorescence of Zfp260-V5 before and after osteogenic induction (left), with the nuclear MFI/cytosolic MFI evaluated (right). n Separation of NE and CE, followed by Western blot. o Representative ARS staining images of PSCs. Scalebar: 2 mm. p–r ChIP-qPCR assays for Zfp260-V5, Brd4, and H3K27ac binding. n = 6 from 2 biological replicates. For ( a , d , e , h , i ), experiments were conducted independently 3 times, consistently producing similar results. For ( c , g , h , i , l , n , o ), n = 3 from 3 biological replicates. For ( f , m ), n = 30 from 3 biological replicates, with 10 randomly selected cells calculated per replicate. For ( e , f , m ), scale bars: 5 μm. Two-way ANOVA. Box plots display the minimum and maximum values, with the center line representing the median, and the bounds of the box representing the 25th to 75th percentiles. Other data in this figure are represented as mean ± SD. Source data and exact p values are provided in the Source Data file.
    Anti Human Mouse Rat Prkca, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+mouse+rat+prkca/PKC+alpha+Antibody+(MC5)/pmc11586402-322-75-78
    Average 92 stars, based on 1 article reviews
    anti human mouse rat prkca - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    a GST-pull down assay of PSC’s whole cell lysate (WCL). The red dotted box indicated the regions for M/S analysis. b GST-Zfp260 specially enriched kinases with high HT sequest scores. c Co-IP of 293 T cell line. d Co-IP of immortalized PSCs. e Co-staining image for Zfp260 and Prkca in PSCs. The white dotted circle indicated the nucleus (Left). The white dotted line indicated the route for the fluorescence intensity measurements (right). f Representative images of immunofluorescence of Zfp260 with osteogenic induction, with the MFI/cytosolic MFI calculated (right). g Separation of nuclear (NE) and cytosolic extracts (CE) followed by Western blot. h In vitro Phos-Assay was performed by Phospho-PAGE. i Co-IP, Phos-PAGE, and SDS-PAGE were jointly performed in immortalized PSCs. The red dotted rectangle indicating the Y173, S182, and S197 residues. j Suggested binding mode of Zfp260 and Prkca by AlphaFold2. k The shortest distance between Zfp260-aa173 and the catalytic domain (CD) of Prkca (Z173-CD), aa182 and CD (Z182-CD), and aa197 and CD (Z197-CD) were calculated. l Co-IP was performed in immortalized PSCs, with statistical analysis (right). m Representative images of immunofluorescence of Zfp260-V5 before and after osteogenic induction (left), with the nuclear MFI/cytosolic MFI evaluated (right). n Separation of NE and CE, followed by Western blot. o Representative ARS staining images of PSCs. Scalebar: 2 mm. p–r ChIP-qPCR assays for Zfp260-V5, Brd4, and H3K27ac binding. n = 6 from 2 biological replicates. For ( a , d , e , h , i ), experiments were conducted independently 3 times, consistently producing similar results. For ( c , g , h , i , l , n , o ), n = 3 from 3 biological replicates. For ( f , m ), n = 30 from 3 biological replicates, with 10 randomly selected cells calculated per replicate. For ( e , f , m ), scale bars: 5 μm. Two-way ANOVA. Box plots display the minimum and maximum values, with the center line representing the median, and the bounds of the box representing the 25th to 75th percentiles. Other data in this figure are represented as mean ± SD. Source data and exact p values are provided in the Source Data file.

    Journal: Nature Communications

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells

    doi: 10.1038/s41467-024-54640-0

    Figure Lengend Snippet: a GST-pull down assay of PSC’s whole cell lysate (WCL). The red dotted box indicated the regions for M/S analysis. b GST-Zfp260 specially enriched kinases with high HT sequest scores. c Co-IP of 293 T cell line. d Co-IP of immortalized PSCs. e Co-staining image for Zfp260 and Prkca in PSCs. The white dotted circle indicated the nucleus (Left). The white dotted line indicated the route for the fluorescence intensity measurements (right). f Representative images of immunofluorescence of Zfp260 with osteogenic induction, with the MFI/cytosolic MFI calculated (right). g Separation of nuclear (NE) and cytosolic extracts (CE) followed by Western blot. h In vitro Phos-Assay was performed by Phospho-PAGE. i Co-IP, Phos-PAGE, and SDS-PAGE were jointly performed in immortalized PSCs. The red dotted rectangle indicating the Y173, S182, and S197 residues. j Suggested binding mode of Zfp260 and Prkca by AlphaFold2. k The shortest distance between Zfp260-aa173 and the catalytic domain (CD) of Prkca (Z173-CD), aa182 and CD (Z182-CD), and aa197 and CD (Z197-CD) were calculated. l Co-IP was performed in immortalized PSCs, with statistical analysis (right). m Representative images of immunofluorescence of Zfp260-V5 before and after osteogenic induction (left), with the nuclear MFI/cytosolic MFI evaluated (right). n Separation of NE and CE, followed by Western blot. o Representative ARS staining images of PSCs. Scalebar: 2 mm. p–r ChIP-qPCR assays for Zfp260-V5, Brd4, and H3K27ac binding. n = 6 from 2 biological replicates. For ( a , d , e , h , i ), experiments were conducted independently 3 times, consistently producing similar results. For ( c , g , h , i , l , n , o ), n = 3 from 3 biological replicates. For ( f , m ), n = 30 from 3 biological replicates, with 10 randomly selected cells calculated per replicate. For ( e , f , m ), scale bars: 5 μm. Two-way ANOVA. Box plots display the minimum and maximum values, with the center line representing the median, and the bounds of the box representing the 25th to 75th percentiles. Other data in this figure are represented as mean ± SD. Source data and exact p values are provided in the Source Data file.

    Article Snippet: The primary antibodies used in mIHC (dilution 1:400 for all antibodies) included goat anti-mouse/human/rat Itgav (AF1219, Novus Biologicals), mouse anti-mouse/rat CD90 (NB100-65543, Novus Biologicals), mouse anti-mouse/human CD105 (NBP2-22122, Novus Biologicals), rabbit anti-human/mouse/rat CD200 (AF2724, Novus Biologicals), rabbit anti-mouse/human/rat Runx2 (ab236639, Abcam), rabbit anti-mouse/human/rat Sox9 (ab185966, Abcam), rabbit anti-mouse/human Alpl (MA5-24845, Invitrogen), rabbit anti-mouse/human/rat Zfp260 (ABE295, Merck), mouse anti-human/mouse/rat p300 (NB100-616, Novus Biologicals), rabbit anti-human/mouse MED1 (NB100-2574, Novus Biologicals), rabbit anti-human/mouse BRD4 (NBP2-76393, Novus Biologicals), mouse anti-human/mouse/rat Prkca (NB600-201, Novus Biologicals), rabbit anti-V5 tag (13202, CST), mouse anti-Collagen type I (67288-1-Ig, proteintech), rabbit anti-Collagen type II (28459-1-AP, proteintech).

    Techniques: Pull Down Assay, Co-Immunoprecipitation Assay, Staining, Fluorescence, Immunofluorescence, Western Blot, In Vitro, SDS Page, Binding Assay, ChIP-qPCR